The genetic properties accompanied of "quasi-species" crossing of Small Ruminant LentiViruses (SRLVs)
نویسندگان
چکیده
The env gene of Small Ruminant LentiViruses (SRLV) encodes a polypeptide precursor which after glycosylation is cleaved to give the two glycoproteins viral envelope: transmembrane protein (TM) and surface (SU). passage adaptation SRLVs in new hosts always accompanied by genetic mutations SU region that determines tropism virus recognition cellular receptor. We studied properties "quasi-species" Capra ibex. 16 Blood samples ibex were tested for presence specific antibodies against using commercially available ELISA. Peripheral blood mononuclear cells (PBMC) isolated on Ficoll gradient cultured macrophage differentiation medium obtain monocyte-derived (MDM) monolayers isolation. DNAs from non PBMC used as templates PCR Nested amplification proviral genome. products (108 nt) cloned sequenced. Sequences analysed ClustalW software. alignments sequences show three important types mutations: deletion, addition replacement nucleotides. present data showing C. SRLV isolates are closer prototypic CAEV-Co isolate. This might indicate recent infection
منابع مشابه
Characterization of Ovine A3Z1 Restriction Properties against Small Ruminant Lentiviruses (SRLVs)
Intrinsic factors of the innate immune system include the apolipoprotein B editing enzyme catalytic polypeptide-like 3 (APOBEC3) protein family. APOBEC3 inhibits replication of different virus families by cytosine deamination of viral DNA and a not fully characterized cytosine deamination-independent mechanism. Sheep are susceptible to small ruminant lentivirus (SRLVs) infection and contain thr...
متن کاملSmall Ruminant Lentiviruses (SRLVs) Break the Species Barrier to Acquire New Host Range
Zoonotic events of simian immunodeficiency virus (SIV) from non-human primates to humans have generated the acquired immunodeficiency syndrome (AIDS), one of the most devastating infectious disease of the last century with more than 30 million people dead and about 40.3 million people currently infected worldwide. Human immunodeficiency virus (HIV-1 and HIV-2), the two major viruses that cause ...
متن کاملRoutes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes.
Small ruminant lentiviruses (SRLV = maedi-visna in sheep and caprine arthritis encephalitis in goats) are distributed throughout most countries of the world, particularly Europe. Laboratories from 16 European countries established collaborations within the framework of a COST (CO-operation in the field of Scientific and Technical Research) action sponsored by the European Union in order to (i) ...
متن کاملImmunization against Small Ruminant Lentiviruses
Multisystemic disease caused by Small Ruminant Lentiviruses (SRLV) in sheep and goats leads to production losses, to the detriment of animal health and welfare. This, together with the lack of treatments, has triggered interest in exploring different strategies of immunization to control the widely spread SRLV infection and, also, to provide a useful model for HIV vaccines. These strategies inv...
متن کاملSmall Ruminant Lentiviruses: Genetic Variability, Tropism and Diagnosis
Small ruminant lentiviruses (SRLV) cause a multisystemic chronic disease affecting animal production and welfare. SRLV infections are spread across the world with the exception of Iceland. Success in controlling SRLV spread depends largely on the use of appropriate diagnostic tools, but the existence of a high genetic/antigenic variability among these viruses, the fluctuant levels of antibody a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ???? ?????? ?????? ??????????
سال: 2021
ISSN: ['2708-8251', '2521-9200']
DOI: https://doi.org/10.51984/jopas.v20i4.1993